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KMID : 0381120200420020155
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Genes and Genomics
2020 Volume.42 No. 2 p.155 ~ p.164
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Population genetic structure and phylogenetic analysis of gray’s pipefish, Halicampus grayi in the South China Sea
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Wu Yingying
Wang Xin
Liu Shuaishuai
Luo Hao
Lin Qiang
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Abstract
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Background: The evolution of male pregnancy is the most distinctive characteristic of syngnathids, and they were recognized as flagship species for marine conservation. Genus Halicampus is an important branch of syngnathid fishes that has not received the attention it deserves.
Objective: To sequence the mitochondrial genome of Halicampus grayi, and investage the genetic structure of its populations.
Methods: Degenerate primers were designed to amplify the entire mitochondrial genome of H. grayi. The phylogenetic relationship between H. grayi and other syngnathids were conducted using maximum-likelihood method. Population genetic structure of three geographic population of H. grayi were determined using median-joining haplotype network based on COI and Cytb sequences.
Results: The complete mitochondrial genome of Halicampus grayi was assembled into a 17,059 bp circular sequence, which contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 D-loop region. The overall base composition of H. grayi is 29.93% A, 29.31% T, 16.23% G and 24.54% C, with a slight A?+?T rich feature (59.24%). Phylogenetic analysis indicated that H. grayi has a close relationship with Trachyrhamphus serratus. Population genetic analysis revealed a relatively high genetic diversity across different geographic populations of H. grayi, and the results of median-joining haplotype network indicated a lack of structure in populations of H. grayi.
Conclusion: The mitogenome of H. grayi will provided important information about the origin and evolution issues of syngnathid fishes, and the high-level genetic diversity detected in their populations will provide insight into the gene flow pattern of marine fishes.
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KEYWORD
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Syngnathidae, Mitochondrial genome, Halicampus grayi, Genetic structure, Phylogenetics
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